In vivo continuous directed evolution.

In vivo continuous evolution of genes and pathways in yeast

Directed evolution remains a powerful, highly generalizable approach for improving the performance of biological systems. However, implementations in eukaryotes rely either on in vitro diversity generation or limited mutational capacities. Here we synthetically optimize the retrotransposon Ty1 to enable in vivo generation of mutant libraries up to 1.6 × 107 l-1 per round, which is the highest o...

Meet-continuity on $L$-directed Complete Posets

In this paper, the definition of meet-continuity on $L$-directedcomplete posets (for short, $L$-dcpos) is introduced. As ageneralization of meet-continuity on crisp dcpos, meet-continuity on$L$-dcpos, based on the generalized Scott topology, ischaracterized. In particular, it is shown that every continuous$L$-dcpo is meet-continuous and $L$-continuous retracts ofmeet-continuous $L$-dcpos are al...

Reproductive Performance of Mouse Oocyte after In Vivo Exposure of The Ovary to Continuous Wave Ultrasound

Continuous dependence on coefficients for stochastic evolution equations with multiplicative Levy Noise and monotone nonlinearity

Semilinear stochastic evolution equations with multiplicative L'evy noise are considered‎. ‎The drift term is assumed to be monotone nonlinear and with linear growth‎. ‎Unlike other similar works‎, ‎we do not impose coercivity conditions on coefficients‎. ‎We establish the continuous dependence of the mild solution with respect to initial conditions and also on coefficients. ‎As corollaries of ...

COVID-19 Tragic Pandemic: Concerns over Unintentional “Directed Accelerated Evolution” of Novel Coronavirus (SARS-CoV-2) and Introducing a Modified Treatment Method for ARDS

Global health authorities are trying to work out the current status of the novel coronavirus (COVID-19) outbreak and explore methods to reduce the rate of its transmission to healthy individuals. In this viewpoint we provide insights concerning how health care professionals can unintentionally shift the novel coronavirus type to more drug-resistant forms. It is worth noting that viruses usually...